Journal: International Journal of Molecular Sciences

Article Title: The Effect of Orally Administered Multi-Strain Probiotic Formulation ( Lactobacillus , Bifidobacterium ) on the Phagocytic Activity and Oxidative Metabolism of Peripheral Blood Granulocytes and Monocytes in Lambs

doi: 10.3390/ijms25105068

Figure Lengend Snippet: Dot plot cytogram showing the percentage of granulocytes stimulated to undergo respiratory burst in control and experimental lambs on experimental day 30. Whole heparinized blood from control group and experimental group (supplemented with the multi-strain probiotic) animals was divided into four test tubes. The samples were combined with the washing solution (negative control), E. coli bacteria (opsonizing stimulus), PMA (strong stimulus) or fMLP (weak stimulus), and incubated with dihydrorhodamine 123 in a water bath at a temperature of 37 °C. After incubation, cells were lysed and the DNA staining solution was added. The percentages of granulocytes stimulated to undergo respiratory burst (conversion of dihydrorhodamine 123 to rhodamine 123) were gated.

Article Snippet: Two hundred microlitres of the DNA staining solution (Glycotope Biotechnology GmbH, Heidelberg, Germany) chilled to 0 °C was added; the samples were shaken and incubated for 10 min in an ice bath.

Techniques: Control, Negative Control, Bacteria, Incubation, Staining

Journal: International Journal of Molecular Sciences

Article Title: The Effect of Orally Administered Multi-Strain Probiotic Formulation ( Lactobacillus , Bifidobacterium ) on the Phagocytic Activity and Oxidative Metabolism of Peripheral Blood Granulocytes and Monocytes in Lambs

doi: 10.3390/ijms25105068

Figure Lengend Snippet: Dot plot cytogram showing the percentage of monocytes stimulated to undergo respiratory burst in control and experimental group lambs on experimental day 30. Whole heparinized blood from control group and experimental group (supplemented with the multi-strain probiotic) animals was divided into four test tubes. The samples were combined with the washing solution (negative control), E. coli bacteria (opsonizing stimulus), PMA (strong stimulus) or fMLP (weak stimulus), and incubated with dihydrorhodamine 123 in a water bath at a temperature of 37 °C. After incubation, cells were lysed and the DNA staining solution was added. The percentages of granulocytes stimulated to undergo respiratory burst (conversion of dihydrorhodamine 123 to rhodamine 123) were gated.

Article Snippet: Two hundred microlitres of the DNA staining solution (Glycotope Biotechnology GmbH, Heidelberg, Germany) chilled to 0 °C was added; the samples were shaken and incubated for 10 min in an ice bath.

Techniques: Control, Negative Control, Bacteria, Incubation, Staining